Schild Plot Generator for Competitive Antagonist

Agonist
Cell
Competitive Antagonist
Effect of Competitive Antagonist on Agonist Dose Response curve
−12−11−10−9−8−7−6−5−4−3−2020406080100
[Agonist] (log M)Effect (% Emax)Level of Effect
Schild Plot
−10−8−6−401234
Log [Antagonist] (log M)Log(DR-1)

Current Level of effect (%Emax) is 50.0%

Questions
Why would you consider conducting a Schild analysis?
Schild Analysis Table

Schild Plot Property Table




Agonist
Cell
Irreversible Antagonist
Effect of Irreversible Antagonist on Agonist Dose Response curve
−12−11−10−9−8−7−6−5−4−3−2020406080100
[Agonist] (log M)Effect (% Emax)Level of Effect
Schild Plot Why might Schild Plots be Non-linear? Click here to learn more
−10−9−8−700.511.52
Actual PlotIdeal PlotLog [Antagonist] (log M)Log(DR-1)

Current Level of effect (%Emax) is 30.0%

Questions
Will the Schild plot for an irreversible antagonist be linear with a slope = 1.0?
Schild Analysis Table

Schild Plot Property Table




Agonist
Cell
Allosteric Antagonist


Effect of Allosteric Antagonist on Agonist-Dose-Response curve
−12−11−10−9−8−7−6−5−4−3−2020406080100
[Agonist] (log M)Effect (% Emax)Level of Effect
Schild Plot Why might Schild Plots be Non-linear? Click here to learn more
−10−8−6−400.511.522.5
Actual PlotIdeal PlotLog [Antagonist] (log M)Log(DR-1)

Current Level of effect (%Emax) is 50.0%

Questions
For an allosteric antagonist that reduces agonist affinity only, will the Schild plot be linear with a slope = 1.0?
Schild Analysis Table

Schild Plot Property Table




Agonist
Cell
Allosteric Antagonist


Effect of Allosteric Antagonist on Agonist-Dose-Response curve
−12−11−10−9−8−7−6−5−4−3−2020406080100
[Agonist] (log M)Effect (% Emax)Level of Effect
Schild Plot Why might Schild Plots be Non-linear? Click here to learn more
−10−9−8−7−600.511.52
Actual PlotIdeal PlotLog [Antagonist] (log M)Log(DR-1)

Current Level of effect (%Emax) is 30.0%

Questions
For an allosteric antagonist that reduces agonist efficacy only, is a Schild plot likely to be linear with a slope equal to one?
Schild Analysis Table

Schild Plot Property Table




Agonist
Cell
Functional Antagonist
Cell
Effect of Functional Antagonist on Agonist Dose Response curve
−12−11−10−9−8−7−6−5−4−3−2020406080100
[Agonist] (log M)Effect (% Emax)Level of Effect
Schild Plot Why might Schild Plots be Non-linear? Click here to learn more
−10−9−8−7−600.511.52
Actual PlotIdeal PlotLog [Antagonist] (log M)Log(DR-1)

Current Level of effect (%Emax) is 30.0%

Questions
Will the Schild plot for a functional antagonist be linear with a slope = 1.0?
Schild Analysis Table

Schild Plot Property Table