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Dose-Response Visualiser
Schild Plot Generator
Schild Plot Analysis Quiz
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Non-Ideal Schild Plots
Schild Plot Generator for Competitive Antagonist
What to do?
Competitive Antagonist
Irreversible Antagonist
Allosteric Antagonist (Affinity)
Allosteric Antagonist (Efficacy)
Functional Antagonist
Agonist
Affinity (∝ 1/K
A
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Competitive Antagonist
Antagonist Affinity (∝ 1/K
B
)
-logK
B
Effect of Competitive Antagonist on Agonist Dose Response curve
−12
−11
−10
−9
−8
−7
−6
−5
−4
−3
−2
0
20
40
60
80
100
[Agonist] (log M)
Effect (% Emax)
Level of Effect
Schild Plot
−10
−8
−6
−4
0
1
2
3
4
Log [Antagonist] (log M)
Log(DR-1)
Reset
Level of effect (%Emax)
Current Level of effect (%Emax) is
50.0
%
Questions
Why would you consider conducting a Schild analysis?
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Answer
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Previous Question
Reveal Answer
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Schild Analysis Table
[Antagonist] (M)
log[B]
[Agonist]
50.0
% Emax
Dose Ratio
log(DR-1)
Schild Plot Property Table
Slope
pA
2
(x-intercept)
R
2
1.00
-9.00
1.00
Agonist
Affinity (∝ 1/K
A
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Irreversible Antagonist
Antagonist Affinity (∝ 1/K
B
)
-logK
B
Effect of Irreversible Antagonist on Agonist Dose Response curve
−12
−11
−10
−9
−8
−7
−6
−5
−4
−3
−2
0
20
40
60
80
100
[Agonist] (log M)
Effect (% Emax)
Level of Effect
Schild Plot
Why might Schild Plots be Non-linear? Click here to learn more
−10
−9
−8
−7
0
0.5
1
1.5
2
Actual Plot
Ideal Plot
Log [Antagonist] (log M)
Log(DR-1)
Reset
Level of effect (%Emax)
Current Level of effect (%Emax) is
30.0
%
Questions
Will the Schild plot for an irreversible antagonist be linear with a slope = 1.0?
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Answer
Restart
Previous Question
Reveal Answer
Next Question
Schild Analysis Table
[Antagonist] (M)
log[B]
[Agonist]
30.0
% Emax
Dose Ratio
log(DR-1)
Schild Plot Property Table
Slope
pA
2
(x-intercept)
R
2
1.45
-9.07
0.98
Agonist
Affinity (∝ 1/K
A
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Allosteric Antagonist
Antagonist Affinity (∝ 1/K
B
)
-logK
B
=
Cooperativity Factor (α)
α =
Effect of Allosteric Antagonist on Agonist-Dose-Response curve
−12
−11
−10
−9
−8
−7
−6
−5
−4
−3
−2
0
20
40
60
80
100
[Agonist] (log M)
Effect (% Emax)
Level of Effect
Schild Plot
Why might Schild Plots be Non-linear? Click here to learn more
−10
−8
−6
−4
0
0.5
1
1.5
2
2.5
Actual Plot
Ideal Plot
Log [Antagonist] (log M)
Log(DR-1)
Reset
Level of effect (%Emax)
Current Level of effect (%Emax) is
50.0
%
Questions
For an allosteric antagonist that reduces agonist affinity only, will the Schild plot be linear with a slope = 1.0?
There are no more questions. Would you like to try again?
Answer
Restart
Previous Question
Reveal Answer
Next Question
Schild Analysis Table
[Antagonist] (M)
log[B]
[Agonist]
50.0
%Emax
Dose Ratio
log(DR-1)
Schild Plot Property Table
Slope
pA
2
(x-intercept)
R
2
0.35
-8.74
0.82
Agonist
Affinity (∝ 1/K
A
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Allosteric Antagonist
Antagonist Affinity (∝ 1/K
B
)
-logK
B
=
Cooperativity Factor (β)
β =
Effect of Allosteric Antagonist on Agonist-Dose-Response curve
−12
−11
−10
−9
−8
−7
−6
−5
−4
−3
−2
0
20
40
60
80
100
[Agonist] (log M)
Effect (% Emax)
Level of Effect
Schild Plot
Why might Schild Plots be Non-linear? Click here to learn more
−10
−9
−8
−7
−6
0
0.5
1
1.5
2
Actual Plot
Ideal Plot
Log [Antagonist] (log M)
Log(DR-1)
Reset
Level of effect (%Emax)
Current Level of effect (%Emax) is
30.0
%
Questions
For an allosteric antagonist that reduces agonist efficacy only, is a Schild plot likely to be linear with a slope equal to one?
There are no more questions. Would you like to try again?
Answer
Restart
Previous Question
Reveal Answer
Next Question
Schild Analysis Table
[Antagonist] (M)
log[B]
[Agonist]
30.0
% Emax
Dose Ratio
log(DR-1)
Schild Plot Property Table
Slope
pA
2
(x-intercept)
R
2
1.18
-9.29
1.00
Agonist
Affinity (∝ 1/K
A
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Functional Antagonist
Affinity (∝ 1/K
B
)
Intrinsic Efficacy (ε)
Cell
Receptor Density (R
T
)
Signal Amplification (
γ
)
Effect of Functional Antagonist on Agonist Dose Response curve
−12
−11
−10
−9
−8
−7
−6
−5
−4
−3
−2
0
20
40
60
80
100
[Agonist] (log M)
Effect (% Emax)
Level of Effect
Schild Plot
Why might Schild Plots be Non-linear? Click here to learn more
−10
−9
−8
−7
−6
0
0.5
1
1.5
2
Actual Plot
Ideal Plot
Log [Antagonist] (log M)
Log(DR-1)
Reset
Level of effect (%Emax)
Current Level of effect (%Emax) is
30.0
%
Questions
Will the Schild plot for a functional antagonist be linear with a slope = 1.0?
There are no more questions. Would you like to try again?
Answer
Restart
Previous Question
Reveal Answer
Next Question
Schild Analysis Table
[Antagonist] (M)
log[B]
[Agonist]
30.0
% Emax
Dose Ratio
log(DR-1)
Schild Plot Property Table
Slope
pA
2
(x-intercept)
R
2
1.39
-8.40
0.99